Download Cell Membrane Transport: Principles and Techniques by Arnošt Kotyk, Karel Janáček (auth.) PDF

By Arnošt Kotyk, Karel Janáček (auth.)

TO the second one version whilst getting ready the manuscript for the unique version of this booklet we have been basically partially conscious of the velocity at which the sector of membrane delivery used to be constructing and at which new rules in addition to new recommendations will be utilized to it. in point of fact that a few of the chapters are actually superseded (e. g. , the only at the molecular facets of delivery) and so on require revision within the mild of recent info that has seemed long ago 5 years. although, it's also precise that we overemphasized within the first version convinced issues that now seem less significant and underestimated the impression of yes others that experience considering that assumed a place one of the so much forcefully mentioned themes of membrane study. In making amends, it was once hence inspiration worthy to incorporate the dialogue of those latter difficulties either within the theoretical and within the comparative sections and, however, to forget a number of the much less topical topics. there has been a special reason behind rewriting the part on kidney and for losing the part on mito­ chondria. assistance from knowledgeable nephrologist was once enlisted for bettering bankruptcy 24, whereas it was once made up our minds that mitochondria characterize a different box either conceptually (being merely subcellular debris) and methodologically (more oblique estimation suggestions being concerned than with complete cells or tissues) and that extra enough info are available in treatises focusing on paintings with mitochondria.

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Extra info for Cell Membrane Transport: Principles and Techniques

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D o2e ot ox 2 (22a) is Fick's second law for unidirectional diffusion. A more general expression in Cartesian coordinates (22b) may be derived analogously. In other systems of coordinates Fick's second law (also called the diffusion equation) will take still other forms but for 37 2. Transport in Homogeneous Liquid Phase all of them the general formula ~= at DL1c = D[72c (22c) can be used, where L1 or [72 is the Laplacian operator. With appropriate initial and boundary conditions and provided that the diffusion coefficient D is considered as a constant the diffusion equation can be solved and functions like c = f(x, y, z, t) can be found.

Equation (10) may be derived as follows. " A steady condition is reached when the driving force and the resistance force acting on the particle are equal (F = Iv) and the particle moves with a constant velocity F V=- I (11) For a spherical particle which is very large as compared with the molecules of the viscous liquid, the "frictional force" is defined by Stokes' formula 1= 6n'YJr (12) 34 Kinetic Aspects and the velocity of the particle will be F v=-- 6n'YJr (13) The force acting on a single particle is equal to the force acting on one mole of the particles (called "total driving force" in Teorell's equation (2», divided by Avogadro's number N F = Total driving force N (14) Moreover, the velocity of particles multiplied by the molar concentration of the substance gives the flux of the substance in moles per unit area per unit time Flux = vc (15) (the amount of substance penetrating across a unit area per unit time will be contained in a cylinder with a base of unit area and a height equal to v).

Golgi membranes of exocrine cells of pancreas with regular globular structures oriented along the plane of section. 05,tm. ) 20 Structural Aspects FIG. 13. Surface view of the plasma membrane in a frozen-etched cell of Saccharomyces cerevisiae. Note the hexagonal arrangement of particles between the invaginations. 5 pm . ) 1. Composition and Structure of Cell Membranes 21 FIG. 14. A hexagonal phospholipid structure in 3% water. The dense spots are hydrophilic "cylinders" surrounded by lipid. 01 pm.

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